Effect of Ruta montana essential oil on Culiseta longiareolata larvae: Toxicity and Biomarkers.

Abstract

This study aims to test the effect of essential oil extracted from Ruta montana on Culiseta longiareolata mosquito species most prevalent in the Tebessa region, The essential oil was obtained by distillation using a Clevenger-type apparatus, showing a yield of 0.57% of the dry matter of the aerial part of the plant. Ruta montana essential oils at different concentrations were tested on Culesita longiareolata mosquito larvae in laboratory conditions according to WHO recommendations. Toxicological tests of Ruta montana show larvicidal and bactericidal activity of these essential oils with a dose-response relationship. Sub-lethal and lethal larval concentrations CL25=3.25 μL/mL, CL90=7.21 μL/ml and CL50=4.24 μL/m The biomarker results reveal an induction of the detoxification system of horseradish essential oil by an increase in GST and a decrease in CAT secretion. The results also showed a significant decrease in the amount of total protein and weight of mosquito larvae.